Nitrogen and sulfur requirements for Clostridium thermocellum and Caldicellulosiruptor bescii on cellulosic substrates in minimal nutrient media.
Identifieur interne : 002572 ( Main/Exploration ); précédent : 002571; suivant : 002573Nitrogen and sulfur requirements for Clostridium thermocellum and Caldicellulosiruptor bescii on cellulosic substrates in minimal nutrient media.
Auteurs : Donna M. Kridelbaugh [États-Unis] ; Joshua Nelson ; Nancy L. Engle ; Timothy J. Tschaplinski ; David E. GrahamSource :
- Bioresource technology [ 1873-2976 ] ; 2013.
Descripteurs français
- KwdFr :
- Azote (métabolisme), Biocarburants (MeSH), Cellobiose (métabolisme), Cellulose (métabolisme), Clostridium thermocellum (croissance et développement), Clostridium thermocellum (métabolisme), Fermentation (MeSH), Milieux de culture (MeSH), Populus (métabolisme), Soufre (métabolisme), Technique d'immunofluorescence (MeSH), Techniques de coculture (MeSH), Vitamines (métabolisme), Éthanol (métabolisme).
- MESH :
- croissance et développement : Clostridium thermocellum.
- métabolisme : Azote, Cellobiose, Cellulose, Clostridium thermocellum, Populus, Soufre, Vitamines, Éthanol.
- Biocarburants, Fermentation, Milieux de culture, Technique d'immunofluorescence, Techniques de coculture.
English descriptors
- KwdEn :
- Biofuels (MeSH), Cellobiose (metabolism), Cellulose (metabolism), Clostridium thermocellum (growth & development), Clostridium thermocellum (metabolism), Coculture Techniques (MeSH), Culture Media (MeSH), Ethanol (metabolism), Fermentation (MeSH), Fluorescent Antibody Technique (MeSH), Nitrogen (metabolism), Populus (metabolism), Sulfur (metabolism), Vitamins (metabolism).
- MESH :
- chemical , metabolism : Cellobiose, Cellulose, Ethanol, Nitrogen, Sulfur, Vitamins.
- chemical : Biofuels, Culture Media.
- growth & development : Clostridium thermocellum.
- metabolism : Clostridium thermocellum, Populus.
- Coculture Techniques, Fermentation, Fluorescent Antibody Technique.
Abstract
Growth media for cellulolytic Clostridium thermocellum ATCC 27405 and Caldicellulosiruptor bescii bacteria usually contain excess nutrients that would increase costs for consolidated bioprocessing for biofuel production and create a waste stream with nitrogen, sulfur and phosphate. C. thermocellum was grown on crystalline cellulose with varying concentrations of nitrogen and sulfur compounds, and growth rate and ethanol production response curves were determined. Both bacteria assimilated sulfate in the presence of ascorbate reductant, increasing the ratio of oxidized to reduced fermentation products. From these results, a low ionic strength, defined minimal nutrient medium with decreased nitrogen, sulfur, phosphate and vitamin supplements was developed for the fermentation of cellobiose, cellulose and acid-pretreated Populus. Carbon and electron balance calculations indicate the unidentified residual fermentation products must include highly reduced molecules. Both bacterial populations were maintained in co-cultures with substrates containing cellulose and xylan in defined medium with sulfate and basal vitamin supplements.
DOI: 10.1016/j.biortech.2012.12.006
PubMed: 23306120
Affiliations:
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Graham</name></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biofuels (MeSH)</term><term>Cellobiose (metabolism)</term><term>Cellulose (metabolism)</term><term>Clostridium thermocellum (growth & development)</term><term>Clostridium thermocellum (metabolism)</term><term>Coculture Techniques (MeSH)</term><term>Culture Media (MeSH)</term><term>Ethanol (metabolism)</term><term>Fermentation (MeSH)</term><term>Fluorescent Antibody Technique (MeSH)</term><term>Nitrogen (metabolism)</term><term>Populus (metabolism)</term><term>Sulfur (metabolism)</term><term>Vitamins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Azote (métabolisme)</term><term>Biocarburants (MeSH)</term><term>Cellobiose (métabolisme)</term><term>Cellulose (métabolisme)</term><term>Clostridium thermocellum (croissance et développement)</term><term>Clostridium thermocellum (métabolisme)</term><term>Fermentation (MeSH)</term><term>Milieux de culture (MeSH)</term><term>Populus (métabolisme)</term><term>Soufre (métabolisme)</term><term>Technique d'immunofluorescence (MeSH)</term><term>Techniques de coculture (MeSH)</term><term>Vitamines (métabolisme)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellobiose</term><term>Cellulose</term><term>Ethanol</term><term>Nitrogen</term><term>Sulfur</term><term>Vitamins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biofuels</term><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Clostridium thermocellum</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Clostridium thermocellum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Clostridium thermocellum</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Cellobiose</term><term>Cellulose</term><term>Clostridium thermocellum</term><term>Populus</term><term>Soufre</term><term>Vitamines</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Coculture Techniques</term><term>Fermentation</term><term>Fluorescent Antibody Technique</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biocarburants</term><term>Fermentation</term><term>Milieux de culture</term><term>Technique d'immunofluorescence</term><term>Techniques de coculture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Growth media for cellulolytic Clostridium thermocellum ATCC 27405 and Caldicellulosiruptor bescii bacteria usually contain excess nutrients that would increase costs for consolidated bioprocessing for biofuel production and create a waste stream with nitrogen, sulfur and phosphate. C. thermocellum was grown on crystalline cellulose with varying concentrations of nitrogen and sulfur compounds, and growth rate and ethanol production response curves were determined. Both bacteria assimilated sulfate in the presence of ascorbate reductant, increasing the ratio of oxidized to reduced fermentation products. From these results, a low ionic strength, defined minimal nutrient medium with decreased nitrogen, sulfur, phosphate and vitamin supplements was developed for the fermentation of cellobiose, cellulose and acid-pretreated Populus. Carbon and electron balance calculations indicate the unidentified residual fermentation products must include highly reduced molecules. Both bacterial populations were maintained in co-cultures with substrates containing cellulose and xylan in defined medium with sulfate and basal vitamin supplements.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23306120</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>17</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>130</Volume><PubDate><Year>2013</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Nitrogen and sulfur requirements for Clostridium thermocellum and Caldicellulosiruptor bescii on cellulosic substrates in minimal nutrient media.</ArticleTitle><Pagination><MedlinePgn>125-35</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2012.12.006</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(12)01860-3</ELocationID><Abstract><AbstractText>Growth media for cellulolytic Clostridium thermocellum ATCC 27405 and Caldicellulosiruptor bescii bacteria usually contain excess nutrients that would increase costs for consolidated bioprocessing for biofuel production and create a waste stream with nitrogen, sulfur and phosphate. C. thermocellum was grown on crystalline cellulose with varying concentrations of nitrogen and sulfur compounds, and growth rate and ethanol production response curves were determined. Both bacteria assimilated sulfate in the presence of ascorbate reductant, increasing the ratio of oxidized to reduced fermentation products. From these results, a low ionic strength, defined minimal nutrient medium with decreased nitrogen, sulfur, phosphate and vitamin supplements was developed for the fermentation of cellobiose, cellulose and acid-pretreated Populus. Carbon and electron balance calculations indicate the unidentified residual fermentation products must include highly reduced molecules. Both bacterial populations were maintained in co-cultures with substrates containing cellulose and xylan in defined medium with sulfate and basal vitamin supplements.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kridelbaugh</LastName><ForeName>Donna M</ForeName><Initials>DM</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6038, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nelson</LastName><ForeName>Joshua</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Engle</LastName><ForeName>Nancy L</ForeName><Initials>NL</Initials></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials></Author><Author ValidYN="Y"><LastName>Graham</LastName><ForeName>David E</ForeName><Initials>DE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>12</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014815">Vitamins</NameOfSubstance></Chemical><Chemical><RegistryNumber>16462-44-5</RegistryNumber><NameOfSubstance UI="D002475">Cellobiose</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>70FD1KFU70</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013455" MajorTopicYN="N">Sulfur</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014815" MajorTopicYN="N">Vitamins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>08</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>12</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>1</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>1</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23306120</ArticleId><ArticleId IdType="pii">S0960-8524(12)01860-3</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2012.12.006</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><noCountry><name sortKey="Engle, Nancy L" sort="Engle, Nancy L" uniqKey="Engle N" first="Nancy L" last="Engle">Nancy L. Engle</name><name sortKey="Graham, David E" sort="Graham, David E" uniqKey="Graham D" first="David E" last="Graham">David E. Graham</name><name sortKey="Nelson, Joshua" sort="Nelson, Joshua" uniqKey="Nelson J" first="Joshua" last="Nelson">Joshua Nelson</name><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Kridelbaugh, Donna M" sort="Kridelbaugh, Donna M" uniqKey="Kridelbaugh D" first="Donna M" last="Kridelbaugh">Donna M. Kridelbaugh</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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